Journal: bioRxiv
Article Title: Protozoal populations drive system-wide variation in the rumen microbiome
doi: 10.1101/2024.12.05.626740
Figure Lengend Snippet: a. Illustration of Epidinium cattanei , the protozoal species most strongly associated with RCT-B, predicted to engage a broad range of CAZymes to degrade dietary plant material. Given the size and activity of E. cattanei their fiber degrading metabolism is predicted to impact the rumen structure and function of RCT-B animals, which was supported via Microarray Polymer Profiling (MAPP) of various hemicellulosic plant fibers, which highlighted differential abundances. In the MAPP inset, diamonds indicate medians, whiskers interquartile ranges, and stars represent uncorrected p- values from Wilcoxon rank sum tests. b. Pathway reconstruction for the Acutalibacteraceae -affiliated RUG762 population, strongly associated with RCT-A, highlighting, sugar fermentation, amino acid (red boxes) metabolism and a partial Wood-Ljungdahl Pathway, which was supported by the associated energy conservation machinery such as the electron-bifurcating hydrogenase (HndABCD, [FeFe] group A), ferredoxin:NAD-oxidoreductase (Rnf) complex, and components of a FoF1 ATP synthase. Bold text indicates differentially abundant metabolites from .
Article Snippet: The resultant NaOH extraction supernatants were diluted sequentially (1/2,1/5,1/5,1/5) in microarray printing buffer (55.2% glycerol, 44% water and 0.8% Triton X-100), and the four dilutions were printed in quadruplet onto nitrocellulose membranes using a non-contact microarray robot (Arrayjet, Roslin).
Techniques: Activity Assay, Microarray, Polymer